Effect of calmodulin antagonist EBB on invasion of human fibrosarcoma cell HT1080.
- Author:
Bing PAN
1
;
Yuan ZHOU
;
Jing QI
;
Dong-sheng XIONG
;
Jie-wen LIU
;
Shu-ling QI
;
Yan-hong CHENG
;
Chun-zheng YANG
;
Hui-fang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Benzylisoquinolines; pharmacology; Calmodulin; antagonists & inhibitors; Cell Line, Tumor; Down-Regulation; Fibrosarcoma; pathology; Humans; Matrix Metalloproteinase 2; biosynthesis; genetics; Matrix Metalloproteinase 9; biosynthesis; genetics; Neoplasm Invasiveness; RNA, Messenger; biosynthesis; genetics
- From: Acta Academiae Medicinae Sinicae 2005;27(3):311-314
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the potential effect of EBB, a calmodulin antagonist, on invasion of human fibrosarcoma cells HT1080.
METHODSThe antitumor effect of EBB was assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Activities of matrix metalloproteinase (MMP)-2 and MMP-9 were measured by Zymogrophy analysis. The mRNA levels, of MMP-2, MMP-9, and tissue inhibitor of metalloproteinases (TIMP)-1 were evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR). Transwell chamber assay was applied to measure the effect of EBB on the invasion of HT1080 cells.
RESULTSCalmodulin antagonist EBB inhibited the proliferation of HT1080 cells with an IC50 of (8.2 +/- 1.2) microg/ml. EBB down-regulated the activities of MMP-2 and MMP-9, and down-regulated the mRNA levels of MMP-2 and MMP-9, while up-regulated the mRNA levels of TIMP-1. The invasive ability of HT1080 cells was decreased to (31.13 +/- 2.265)%, (59.91 +/- 2.566)%, and (71.58 +/- 0.5960)% after exposure of the cells with 2, 5, and 10 microg/ml EBB, respectively.
CONCLUSIONTreatment with calmodulin antagonist EBB is effective in suppressing tumor invasion. The possible mechanism is the down-regulation of MMPs.
