Effect of the positive sera of autoantibodies against the human beta1-adrenoceptor from patients with congestive heart failure on activity of L-type Ca2+ channel in guinea pig cardiac myocytes.
- Author:
Shu-yan WANG
1
;
Xin-chun YANG
;
Guo-bin MIAO
;
Xiu-lan LIU
;
Lin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autoantibodies; blood; Calcium Channels, L-Type; metabolism; Female; Guinea Pigs; Heart Failure; immunology; Male; Myocytes, Cardiac; metabolism; Patch-Clamp Techniques; Receptors, Adrenergic, beta; immunology
- From: Acta Academiae Medicinae Sinicae 2005;27(3):332-336
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of the positive sera of autoantibodies against the human beta1-adrenoceptor from patients with congestive heart failure on activity of L-Type Ca2+ channel in guinea pig cardiac myocytes.
METHODUsing whole cell patch-clamp technique, we quantitatively researched the ionic intensity and density of L-type Ca2+ channel (ICa-L).
RESULTSThe beta-adrenocepter agonist isoprenaline increased the ICa-L peak intensity and density from (997.09 +/- 227.5) pA and (8.20 +/- 0.86) pA/pF to (2241.01 +/- 348.5) pA and (18.98 +/- 1.18) pA/pF, respectively (P < 0.01). The positive sera of autoantibodies against the beta1-adrenoceptor could also increase ICa-L peak intensity and density from (963.57 +/- 207.56) pA and (8.14 +/- 0.72) pA/pF to (1382.41 +/- 241.36) pA and (11.70 +/- 1.03) pA/pF (P < 0.01). Esmolol, a beta1-adrenoceptor antagonist blocked these effects of isoprenaline and autoantibodies.
CONCLUSIONSHuman cardiac positive sera of autoantibodies against the beta1-adrenoceptor has an isoproterenol-like effect on cardiac myocytes receptor. It may participate in the pathophysiologic process of cardiac myocytes.
