Inductive effect of zinc oxide nanoparticles on interleukin 8 gene expression in human bronchial epithelial cells and its regulatory mechanism.
- Author:
Yang LU
1
;
Lei XU
;
Zhen YAN
;
Yi-ming WU
;
Wei-dong WU
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; drug effects; Cell Line; Epithelial Cells; drug effects; metabolism; Gene Expression; Humans; Interleukin-8; genetics; metabolism; Nanoparticles; RNA Stability; drug effects; RNA, Messenger; genetics; Zinc Oxide; adverse effects
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(2):117-120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clarify the effect of zinc oxide nanoparticles (ZnO-NPs) (30 nm in diameter) on the interleukin 8 (IL-8) gene expression in human bronchial epithelial cells (BEAS-2B) and its regulatory mechanism.
METHODSBEAS-2B cells were used in the study. The MTT assay was employed to evaluate the damage to BEAS-2B cells by ZnO-NPs. RT-PCR and ELISA were used to measure the mRNA and protein expression levels of IL-8 in the BEAS-2B cells exposed to ZnO-NPs. The IL-8 mRNA decay assay was used to determine the effect of ZnO-NPs on IL-8 mRNA stability.
RESULTSExposure to ZnO-NPs significantly increased the level of IL-8 mRNA in BEAS-2B cells and the level of IL-8 protein in supernatant medium. The transcription inhibitor significantly reduced the mRNA expression of IL-8 induced by ZnO-NPs. ZnO-NPs significantly delayed IL-8 mRNA degradation in the BEAS-2B cells that were pretreated with actinomycin D for terminating IL-8 mRNA synthesis.
CONCLUSIONZnO-NPs can increase the mRNA and protein expression levels of IL-8 and IL-8 mRNA stability in BEAS-2B cells.
