Influence of ethylbenzene on oxidative damage and apoptosis in rat renal epithelial cells NRK-52e.
- Author:
Xiao-ting LIU
1
;
De-yi YANG
;
Yan-rang WANG
;
Qian WANG
;
Dan KUANG
;
Ming ZHANG
;
Li-jie QIAO
;
Jian-guo LI
;
Xue-ying YANG
;
Shu-lan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Benzene Derivatives; toxicity; Cell Line; Epithelial Cells; drug effects; metabolism; Kidney Tubules; cytology; Oxidation-Reduction; Oxidative Stress; drug effects; Rats; Reactive Oxygen Species; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(2):133-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the oxidative damage and apoptosis of renal tubular epithelial cells (NRK-52e cell line) induced by ethylbenzene.
METHODSNRK-52e cells were exposed to 30, 60, 90, 120 μmol/L ethylbenzene for 24 hours. Cell viability were measured using MTT, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), the contents of malondialdehyde (MDA) and glutathione (GSH) were detected respectively. PI fluorescent staining assay was applied to detect percentage of apoptosis in ethylbenzene-treated groups.
RESULTSCompared with control group, cell outline became clear, cell diopter increased, cell became smaller and shrinkage, some cells broke in 60 μmol/L ethylbenzene-treated group. Plenty of cells died, suspension cells increased significantly in 90 μmol/L ethylbenzene-treated group. Compared with control group, cell viability the activities of SOD and CAT and the content of GSH were significantly decreased in 60 and 90 μmol/L ethylbenzene-treated groups (P<0.05). The MDA content were remarkably elevated in 90 μmol/L ethylbenzene-treated groups (P<0.05).
CONCLUSIONEthylbenzene can induce oxidative stress and apoptosis in NRK-52e cells (P<0.05).