Paraquat involves differentiation of human neural stem cells via Notch signaling.
- Author:
Xiu-Li CHANG
1
;
Wen LU
;
Ting-ting DOU
;
Xia SUN
;
Zhi-jun ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Calcium-Binding Proteins; metabolism; Cell Differentiation; drug effects; Cells, Cultured; Embryonic Stem Cells; cytology; drug effects; metabolism; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Jagged-1 Protein; Membrane Proteins; metabolism; Neural Stem Cells; cytology; drug effects; metabolism; Paraquat; pharmacology; Receptor, Notch1; metabolism; Serrate-Jagged Proteins; Signal Transduction; drug effects; Ubiquitin-Protein Ligases; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(7):492-495
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate effects of paraquat on the mRNA expression of key elements of Notch signaling (Notch1, Jagged1 and DTX1) during differentiation process of human neural stem cells (hNSCs).
METHODShNSCs exposed to PQ at the concentrations 0.10, 1.00, 10.00 M. Cell proliferation ability was assessed using MTT assay and mRNA expressions of Notch1, Jagged1 and DTX1 were detected by Real-time RT-PCR at 2, 4, 8, 12 d of differentiation.
RESULTSCompared with control group, NOTCH1, JAG1 mRNA expression levels exposed to PQ at the concentration of 0.10 M significantly reduced at 2, 4, 8 d and significantly went up at 12d (P < 0.01). Compared with control group, NOTCH1, JAG1 and DTX1 mRNA expression levels exposed to PQ at the concentration of 10.00 M significantly reduced at 2, 8, 12 d (P < 0.01). PQ could down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the early stage of differentiation, then up-regulate Notch1 mRNA expression, and down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the end of differentiation.
CONCLUSIONNotch signaling pathway may be involved in differentiation of neural stem cell exposed to PQ.
