Development of radioimmunoassay for human sperm protein 17.
- Author:
Guo-hong WANG
1
;
Fang-qiu LI
;
Wei SUN
;
Rui-ji XU
;
Zhong-shu ZHANG
;
Xiao-jie WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Surface; blood; immunology; Carrier Proteins; blood; immunology; Female; Humans; Rabbits; Radioimmunoassay; methods; Recombinant Proteins; immunology; Reproducibility of Results; Sensitivity and Specificity
- From: National Journal of Andrology 2006;12(6):510-515
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.
METHODSAnti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.
RESULTSThe assay range was 3.3 to approximately 800 microg/L, the sensitivity was 2.0 microg/L, and the intra- and inter-assay coefficients of variation (CV) were 7.5% to approximately 9.8% and 8.2% to approximately 13.2%, respectively. The serum Sp17 level in normal subjects was (15.60 +/- 7.66) microg/L (n = 59).
CONCLUSIONThis radioimmunoassay of Sp17 fulfills the reasonable requirements of clinical routine and scientific studies in terms of specificity, sensitivity and practicability. Measurement of Sp17 concentration is useful for assessing its native distribution and aberrant expression.
