Mechanism of IL-6 on acrosome reaction in human sperm.

Author: Jie ZI ¹ ; Ping SONG
Author Information

1. Clinical Laboratory, Shenzhen Futian Women and Children Health Care Hospital, Shenzhen, Guangdong 518026, China. zijie20@sohu.com
Publication Type:Journal Article
MeSH: Acrosin; metabolism; Acrosome Reaction; drug effects; Adult; Calcium; pharmacology; Cells, Cultured; Humans; Interleukin-6; pharmacology; Male; Naphthalenes; pharmacology; Protein Kinase C; antagonists & inhibitors; Spermatozoa; drug effects; enzymology; Superoxide Dismutase; metabolism
From:National Journal of Andrology 2006;12(6):528-530
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo explore the mechanism of interleukin-6 (IL-6) on acrosome reaction (AR) in human sperm.
METHODSSperm acrosin activity was measured by BAEE/ADH and AR evaluated by FITC-PSA.
RESULTSIL-6 could induce the activity of acrosin and superoxide dismutase (SOD) and enhance AR in human sperm. AR was not induced by extracellular Ca2+, and IL-6-induced AR did not occur in Ca2(+)-free medium. Calphostin C, one inhibitor of the protein kinase C (PKC), could block IL-6-induced AR in human sperm.
CONCLUSIONIL-6 could induce AR by stimulating the activity of acrosin and SOD in human sperm, which also involves the activation of PKC, and requires the presence of extracellular Ca2+.