Castration affects the expression of androgen receptor and epidermal growth factor in rat submaxillary salivary gland.
- Author:
Yun-feng ZHU
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Epidermal Growth Factor; biosynthesis; Male; Orchiectomy; Random Allocation; Rats; Rats, Wistar; Receptors, Androgen; biosynthesis; Submandibular Gland; metabolism
- From:National Journal of Andrology 2006;12(6):540-544
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of castration on the quantity of androgen receptor (AR) and the epidermal growth factor (EGF) in the submaxillary salivary glands of castrated rats.
METHODSSixty male Wistar rats, aged 30 to approximately 60 days, were randomly divided into three groups of 20 rats: castrated, sham-operated and normal control (unoperated). The submaxillary salivary glands of the castrated rats were removed one week after surgery, and so were those of the rats in the sham-operated and the normal control groups. The homogenized salivary gland was used for Western-blots.
RESULTSThe quantity of AR and EGF in the submaxillary salivary glands was decreased significantly in the castrated rats compared with that in the sham-operated and the normal control ( P < 0. 05). Moreover, EGF secreted by the submaxillary salivary glands was also decreased in the castrated rats (P < 0.05).
CONCLUSIONCastration affected the production of androgen receptor in the salivary gland, and also, could further affect the secretion of EGF from the salivary gland.
