Application of distilled water in sperm counting and hypoosmotic swelling test.

Fang Chen; Jin-chun LU; Hui-ru XU; Yu-feng Huang; Yu-an HU; Yong Shao

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Application of distilled water in sperm counting and hypoosmotic swelling test.

Author: Fang CHEN ¹ ; Jin-chun LU ; Hui-ru XU ; Yu-feng HUANG ; Yu-an HU ; Yong SHAO
Author Information

1. Clinical School, Nanjing University Medical College, Department of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Publication Type:Journal Article
MeSH: Adult; Humans; Male; Osmotic Pressure; Sperm Count; Spermatozoa; physiology; Water
From: National Journal of Andrology 2006;12(6):542-544
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo evaluate the application of distilled water in sperm-counting and hypoosmotic swelling test.
METHODSThirty-seven semen samples were collected and each was diluted by distilled water and sodium acid carbonate-formaldehyde solution, respectively. Then the hemacytometer was used for sperm counting. Meanwhile, the percentage of swelled sperm diluted by distilled water was compared with the result of hypoosmotic swelling test recommended by WHO. Another 26 semen samples were diluted by distilled water and hypoosmotic swelling solution respectively, and the percentages of the swelled sperm were compared.
RESULTSThere was no significant difference either between the sperm concentrations obtained by distilled water and sodium acid carbonate-formaldehyde solution (P > 0.05) or between the percentages of the swelled sperm diluted by distilled water and hypoosmotic swelling solution.
CONCLUSIONDistilled water can not only replace sodium acid carbonate-formaldehyde solution for sperm-counting dilution but also be used as a hypoosmotic swelling solution.