Expression of multi-copy and immuno-reactivity of recombinant type-specific epitope of herpes simplex virus type 1.
- Author:
Xiao-wei JI
1
;
Xu-hu MAO
;
Quan-ming ZOU
;
Qing-tan YU
;
Li-li ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Antigen-Antibody Reactions; DNA, Recombinant; Epitopes; biosynthesis; immunology; Herpesvirus 1, Human; immunology; Herpesvirus 2, Human; immunology; Humans; Recombinant Fusion Proteins; biosynthesis; immunology; Viral Envelope Proteins; biosynthesis; immunology
- From: National Journal of Andrology 2006;12(7):579-582
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express a multi-copy specific epitope recombinant protein of herpes simplex virus type 1 (HSV-1) with immuno-reactivity.
METHODSMulti-copy genes with a specific epitope of HSV-1-glycoprotein G 112-127 were constructed by DNA recombination and cloned in E. coli JM109 pGEM-5Zf. The positive recombinants were determined by SDS-PAGE and Western blotting.
RESULTSThe recombinants with 4, 8, 16 and 32 copies of gG 112-127 were obtained. The 8-copy recombinant was expressed by 17.5%, mainly as inclusion body. And it reacted with antiserum HSV-1, but not with antiserum HSV-2.
CONCLUSIONThe HSV-1-gG112-127 recombinant could be used to distinguish HSV-1 and HSV-2 in ELISA.
