Effects of flunarizine on T-type calcium channels in mouse spermatogenic cells.
- Author:
Chang-song WANG
1
;
Xiao-hua GAO
;
Hong CHENG
;
Wei GONG
;
Xi-chun ZHANG
;
Jie CHENG
;
Rong GAO
;
Hang XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium Channel Blockers; pharmacology; Calcium Channels, T-Type; drug effects; physiology; Cells, Cultured; Dose-Response Relationship, Drug; Flunarizine; pharmacology; Male; Mice; Mice, Inbred ICR; Patch-Clamp Techniques; Spermatids; drug effects; physiology
- From: National Journal of Andrology 2006;12(7):594-601
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells.
METHODSCa2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed.
RESULTSFlu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively.
CONCLUSIONFlu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells.
