Evaluation of sperm mitochondrial function using Rh123/PI dual fluorescent staining.
- Author:
Yong-ming WU
1
;
Xin-yi XIA
;
Lian-jun PAN
;
Yong SHAO
;
Bao-fang JIN
;
Yu-feng HUANG
;
Xiu-lai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Flow Cytometry; Humans; Male; Mitochondria; physiology; Rhodamine 123; Sperm Count; Sperm Motility; Spermatozoa; Staining and Labeling; methods
- From: National Journal of Andrology 2006;12(9):803-806
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility and clinical significance of detecting sperm mitochondrial function by using Rh123/PI dual fluorescent staining and flow cytometry analysis, and to explore the relationship between the results of Rh123/PI dual fluorescent staining and seminal parameters.
METHODSSixty-three semen samples were classified as normal (n=31) and abnormal (n=32) according to the World Health Organization guidelines. Rh123/PI dual fluorescent staining was then carried out to evaluate sperm mitochondrial function by flow cytometry analysis.
RESULTSSignificant differences in Rh123+ PI-, Rh123- /PI+ and Rh123- /PI- were detected between the normal and abnormal semen samples (P < 0.05). There was a significant positive correlation between the Rh123+ PI- sperm and sperm motility and a significant inverse correlation between Rh123+ PI- and immotile sperm. But the Rh123- PI+ sperm showed a contrary relationship with Rh123+ PI-. A significant inverse correlation was also observed between the Rhl23- /PI- sperm and sperm concentration in the abnormal group.
CONCLUSIONRh123/PI dual fluorescent staining and flow cytometry analysis can readily and quickly detect sperm mitochondrial function and be used to evaluate semen quality.
