Inhibitory effect of Typhonium gigantewm Engl. on in vitro cultured human keloid fibroblasts.
- Author:
Jing-Wei LÜ
1
;
Gang HU
1
;
Fang LI
1
;
Jia-Jing WANG
1
;
Wei YANG
1
;
Hui HUANG
1
;
Jing-lan LIU
1
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Fibroblasts; cytology; drug effects; pathology; Humans; Keloid
- From: Chinese Journal of Plastic Surgery 2013;29(5):365-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibitory effect of Typhonium gigantewm Engl. (AEoTGE) on the proliferation and apoptosis of KFB in vitro and to survey the death rate.
METHODSSamples of hypertrophic scars were collected and cultured. Only 4-8 passage cells were selected for experiment. Inverted microscope and transmission electron microscope were used to observe the morphogenesis and ultrastructure of KFB. The KFB cells were treated with AEoTGE in different concentrations(3. 125,6.250, 12.500, 25.000, 50. 000,100.000 g/L) for 24 hours. The effect of AEoTGE on the proliferation and the IC50 of KFB was observed with MTT assay and EdU. The effect of AEoTGE on apoptosis of KFB was detected by flow cytometry.
RESULTSIt showed that AEoTGE could inhibit the proliferation of KFB in an concentration-dependent style within the range of 3. 125-100.000 g/L. The AEoTGE could obviously increase the apoptosis rate of the KFB compared with blank control group(P <0.05). The IC50 of AEoTGE was 35 g/L. FITC-Annexin V/PI showed that apoptosis rate of KFB in the AEoTGE group was (72. 07 +/- 0. 70)% , while it was 23. 5% in blank control group (P < 0. 05).
CONCLUSIONSAEoTGE could significantly inhibit the proliferating activity and induce apoptosis of KFB after co-culture for 24 hours. The IC50 is 35 g/L and the rate of apoptosis is (72.07 +/- 0.70)%.
