The influence of zi-hua burn cream on the survival of random skin flaps in rats.
- Author:
Bin-yu LIU
1
;
Bin-yan LIU
1
;
Li-fen LI
1
;
Yin-fei HE
1
;
Ya-rong WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; pharmacology; Graft Survival; drug effects; Interleukin-6; blood; Male; Malondialdehyde; blood; Rats; Rats, Wistar; Skin Transplantation; Superoxide Dismutase; blood; Surgical Flaps; Tumor Necrosis Factor-alpha; blood
- From: Chinese Journal of Plastic Surgery 2013;29(5):370-375
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of zi-hua burn cream on the survival of skin flaps in rats, and its mechanisms.
METHODS72 Wistar rats, were randomly divided into four groups as zi-hua group(n = 18, external application of alfalfa burn cream), control group (n = 18, external application of heparin sodium cream), model group (n = 18, external application of vaseline) , negative control (n = 18, no operation). 8 cm x 2 cm random skin flaps with pedicle on the side of head were designed on the back of Wistar rats. The drug was applied on the flap surface, 2 times a day. The survival of skin flaps was observed. The change of serum superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), turner necrosis factor-alpha(TNF-alpha)and interleukin-6(IL-6)were compared at 1,2,3,7 d after operation, and histologic examination was performed.
RESULTSThe survival rate of zi-hua group (73.58 - 10. 74)% was significantly higher than that of model group (33.40 - 16.05) %, showing a statistical difference (Q = 10.63, P <0.01). There was no significant difference between the zi-hua group and control group (71.65 +/- 11. 92) %. The level of serum SOD, NO in zi-hua group and control group was higher than that in model group, while the level of serum MDA, TNF-alpha and IL-6 was lower than that in model group(P <0.01). On 7 day after operation, skin flaps tissue edema,necrosis and inflammatory cell infiltration in zi-hua group and control group was less obvious than that in model group. There was significant proliferation of granuloma and fibroblast and formation of neonatal capillary in zi-hua group and control group. The vascular density in zi-hua group was obviously higher than that in the model group and control group(P <0. 01).
CONCLUSIONSZi-hua burn cream could significantly improve the blood supply of skin flaps, increase the survival rate of skin flaps in rats. Its mechanism may be associated with the anti-free-radical-damage action, improve local microcirculation, improve the NO content, reduce the TNF-alpha and IL-6 level, reduce inflammation factor release, improve oxidative stress state, and reduce inflammation reaction.
