Effects of botulinum toxin type A on the expression of alpha-SMA and myosin-II of fibroblasts in scars.
- Author:
Tongtong YAN
;
Minliang CHEN
;
Kui MA
;
Liming LIANG
;
Chang LIU
;
Linying LAI
;
Xiaobing FU
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Botulinum Toxins, Type A; pharmacology; Cicatrix; Fibroblasts; drug effects; metabolism; Humans; Muscle, Smooth; metabolism; Myosin Type II; metabolism; Random Allocation
- From: Chinese Journal of Plastic Surgery 2014;30(2):118-121
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of botulinum toxin type A (BTXA) on the expression of alpha smooth muscle actin(alpha-SMA) and myosin-II of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture. Cells from passages 3-5 were randomly divided into 3 groups (control group, low BTXA group (1 U/10(6) Cells), and high BTXA group (2.5 U/ 10(6)Cells)). Growth condition of fibroblasts was observed at 1 , 4, 7 day after BTXA treated. Changes of alpha-SMA and myosin-II in fibroblasts were detected by Western blot.
RESULTSFibroblasts grew well in control group. The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic cells were seen in high BTXA group at 7th day. Proteins of alpha-SMA and myosin-II in fibroblasts were statistically different between BTXA group and control groups at 4th day (P < 0.05). The expression of alpha-SMA and myosin-II in low BTXA group was higher than that in high BTXA group at 7th day (P < 0.05).
CONCLUSIONSBTXA could induce the apoptosis of fibroblasts and decrease the expression of alpha-SMA and myosin-II in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.