Experimental study of skin wound healing with epidermal stem cells from human hypertrophic scar in nude mice.
- Author:
Shuping ZHOU
;
Zhilong HUANG
;
Jiguang MA
;
Jingjing XU
;
Jinglong CAI
;
Xianlei ZONG
;
Le DU
- Publication Type:Journal Article
- MeSH: Animals; Cicatrix, Hypertrophic; pathology; Epidermis; cytology; Female; Humans; Male; Mice; Mice, Nude; Skin; injuries; Stem Cell Transplantation; Stem Cells; Wound Healing
- From: Chinese Journal of Plastic Surgery 2014;30(4):289-293
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of epidermal stem cells from human hypertrophic scar (HS-ESCs) on the skin wound healing in nude mice.
METHODS40 mice were randomly divided into two groups as experimental group (n = 20) and control group (n = 20). Wounds, 1 cm in diameters, were made on every mouse back. The wounds were treated with HS-ESCs and erythromycin ointment in experimental group, or only with erythromycin ointment in control group. The wound healing was observed during the following 14 days. The expression of collagen-I, collagen-III, epidermal growth factor (EGF), fibroblast growth factor (FGF2) , transforming growth factor (TGFbeta1, and TGFbeta2) were studied.
RESULTSThe wound healing time in the experimental group was (20.8 +/- 0.84) d, which was (25.6 +/- 0.89) d in the control group. HE staining revealed that the extent of vascularization in the experimental group was 11.60 +/- 0.55, while it was 8.04 +/- 0.33 in the control group. Immunochemistry analysis showed the expression of collagen-I, collagen-III, EGF, FGF2, TGFbeta1, and TGFbeta2 in the experimental group were significantly higher, compared with those in control group (P < 0.05).
CONCLUSIONHS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.