Effects of propranolol on proliferation of hemangioma-derived mesenchymal stem cells .
- Author:
Zhao TINGHUI
;
Ma XIAORONG
;
Huang YINGYING
;
Chen HUIPING
;
Xiao YAN
;
Ouyang TIANXIANG
- Publication Type:Journal Article
- MeSH: Adipogenesis; Antigens; metabolism; Cell Differentiation; Cell Proliferation; drug effects; Cells, Cultured; Endothelium, Vascular; cytology; Fibroblasts; cytology; Hemangioma; pathology; Humans; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Osteogenesis; Propranolol; pharmacology; Umbilical Veins; Vimentin; metabolism
- From: Chinese Journal of Plastic Surgery 2014;30(5):373-377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the new mechanism of propranolol for treatment of hemangioma and the effects of propranolol on proliferation of hemangioma-derived mesenchymal stem cells ( Hem- MSCs).
METHODSWe isolated Hem-MSCs from hemangioma in the proliferating phase by their selective adhesion to plastic culture dishes. Immunofluorescence staining was used to examine the expression of marker antigens in Hem-MSCs. Human umbilical vein endothelial cells(HUVECs) were used as control. Indiuction of multi-lineage differentiation including osteogenesis and adipogeneis was performed with appropriate medium to identify the multi-lineage differentiation potential. MTT cell counting was used to observe the effects of different concentrations of propranolol on proliferation of Hem-MSCs.
RESULTSHem- MSCs were fibroblast-like morphology. All of them expressed vimentin, most expressed α-SMA,CD133, some expressed Glutl, and none of them expressed VEGF. Osteogenic, adipogenic differentiations of Hem- MSCs were induced successfully. Effects of low concentration of propranolol on proliferation of Hem-MSCs were not obvious, while high concentration of propranolol can inhibit the proliferation of Hem-MSCs.
CONCLUSIONSThe cells we isolated from hemangioma are Hem-MSCs. High concentration of propranolol can inhibit the proliferation of Hem-MSCs.