Induction of CuZn-SOD mRNA expression and activity by PGMS in rat liver.
- Author:
Xiao-ke HU
1
;
Wen-gong YU
;
Xin-zhi LU
;
Feng HAN
;
Qian-hong GONG
;
Yan GAO
;
Hua-shi GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dose-Response Relationship, Drug; Free Radical Scavengers; pharmacology; In Vitro Techniques; Liver; drug effects; metabolism; Male; Propylene Glycols; pharmacology; RNA, Messenger; biosynthesis; drug effects; genetics; Rats; Rats, Wistar; Superoxide Dismutase; biosynthesis; genetics; metabolism
- From: Acta Pharmaceutica Sinica 2002;37(1):23-26
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of propylene glycol mannate sulfate (PGMS) on induction of CuZn-SOD.
METHODSWistar rats were given PGMS p.o. at different doses (0, 18.9, 37.8 and 75.6 mg.kg-1.d) for ten days. Then the rats were sacrificed and the total RNA was extracted from the livers. The total RNA samples were loaded on a 1% agarose gel to detect the quality of total RNA. RT-PCR was applied to study the expression of CuZn-SOD mRNA in rat livers. The amplified products were detected by the 1.5% agarose gel electrophoresis. Simultaneously, the CuZn-SOD activities in rat liver were determined by nitrite method.
RESULTSThe total RNA extracted from rat livers was integrated without being decomposed by RNase. The level of CuZn-SOD mRNA of the high-dosage group (75.6 mg.kg-1.d) was higher than that of the control group (0 mg.kg-1.d) (P < 0.01); the CuZn-SOD activities of the high-dosage group were significantly higher than those of the control group (P < 0.001) and the CuZn-SOD activities of the middle- (37.8 mg.kg-1.d) and low-dosage groups (18.9 mg.kg-1.d) were higher than those of the control group (P < 0.01).
CONCLUSIONPGMS can increase the CuZn-SOD activities as well as CuZn-SOD on mRNA level. Therefore, it is possible for PGMS to counteract Atherosclerosis (AS) by inducing the expression of CuZn-SOD.
