Study on the chiral separation of securinine by high-performance capillary electrophoresis and its stereoselective metabolism in rat.
- Author:
Xiao-hai LI
1
;
Jin-lan ZHANG
;
Tong-hui ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; chemistry; isolation & purification; metabolism; urine; Animals; Azepines; chemistry; isolation & purification; metabolism; urine; Bile; metabolism; Electrophoresis, Capillary; methods; Euphorbiaceae; chemistry; Heterocyclic Compounds, 4 or More Rings; chemistry; isolation & purification; metabolism; urine; Heterocyclic Compounds, Bridged-Ring; Lactones; chemistry; isolation & purification; metabolism; urine; Male; Molecular Structure; Piperidines; chemistry; isolation & purification; metabolism; urine; Plants, Medicinal; chemistry; Rats; Rats, Wistar; Stereoisomerism
- From: Acta Pharmaceutica Sinica 2002;37(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish a high-performance capillary electrophoresis (HPCE) chiral separation method for d-securinine and l-securinine, and use this method to investigate the stereoselective metabolism process of d- and l-securinine in Wistar rats.
METHODSThe electrophoretic condition and parameters were investigated and the optimized conditions were as following: the electrophoretic medium was 40 mmol.L-1 Tris-H3PO4 buffer (pH adjusted to 6.0 with H3PO4) containing 32 mmol.L-1 HP-beta-CD as chiral selector. Determination was carried out with a UV detector at 254 nm. The separations were performed at 16 degrees C with a positive voltage of 15 kV. Samples were injected into the capillary by pressure for 6 s. The biological samples (urine, bile, plasma and feces) of rats were alkalized and extracted with ethyl acetate.
RESULTSThe experimental results showed that the concentration of HP-beta-CD, the concentration of the running buffer and the pH value of the buffer were the main important factors which effected the resolution. d-Securinine and l-securinine were separated at baseline level under the determination conditions. The determination was not interfered by endogenous components and metabolites. After i.p. administration, the rats excreted more d-securinine than l-securinine through bile, urine and feces. The metabolism process in rats was stereoselective.
CONCLUSIONThis method is simple, reliable and suitable for studying the stereoselective metabolism of securinine in rats.