Discrimination of anticancer agent action loci at G(2) and M phases by flow cytometry and confocal microscopic imaging.
- Author:
Yi-Sheng ZHONG
1
;
Chang-Chuan PAN
;
Chang-Nan JIN
;
Jian-Jun LIN
;
Gong-Peng XIONG
;
Jian-Xi ZHANG
;
Jian-Pin GONG
Author Information
1. Department of Hepatic Surgery, Xiamen Municipal Hospital of Chinese Traditional Medicine, Xiamen 361009, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Cell Cycle;
drug effects;
Cell Division;
drug effects;
Flow Cytometry;
G2 Phase;
drug effects;
Humans;
Microscopy, Confocal;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2009;17(4):965-968
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to evaluate a method to discriminate the action loci of anticancer agents in G(2) and M phases of cell cycle. The meta-amsacrine (m-AMSA) and vinblastine (VBL), already known as G(2) and M phase arrest agent respectively, were used to induce the arrest of MOLT-4 cells at G(2) and M phases, the change of DNA content was detected by flow cytometry, the morphology of arrested cells was observed by confocal microscopy so as to find the arrest efficacy difference of 2 anticancer agents. As a result, the flow cytometric detection showed that the arrested MOLT-4 cells displayed the raise of peaks in G(2) and M phases, but flow cytometric detection alone can not discriminate the difference between them. The observation with confocal microscopy showed that the MOLT-4 cells arrested by m-AMSA displayed the morphologic features in G(2) phase, while the MOLT-4 cells arrested by VBL displayed the morphologic features in M phase. This observation with confocal microscopy is helpful to discriminate the difference between them. In conclusion, the combination of flow cytometry with confocal microscopy is one of the effective methods to discriminate the kind of G(2) or M phase arresting agent of anticancer drugs.
