Activated T cells promote differentiation of mesenchymal stem cells into osteoblasts.
- Author:
Yuan-Lin LIU
1
;
Xiao-Xia JIANG
;
Yong-Feng SU
;
Si-Wei HUO
;
Heng ZHU
;
Ying WU
;
Xiu-Sen LI
;
Ning MAO
;
Yi ZHANG
Author Information
1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cells, Cultured;
Coculture Techniques;
Culture Media, Conditioned;
Humans;
Interleukin-1beta;
biosynthesis;
Mesenchymal Stromal Cells;
cytology;
Osteoblasts;
cytology;
T-Lymphocytes;
metabolism
- From:
Journal of Experimental Hematology
2009;17(4):974-976
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1beta played an important role in the effect of activated T cells on MSCs, while TNF-alpha, TGF-beta1 were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interactive influence between MSCs and immune cells can be mediated through cytokines.
