Organ-specific T cell receptor repertoire in target organs of murine graft-versus-host disease after haploidentical bone marrow transplantation.
- Author:
Yue-Wen FU
1
;
De-Pei WU
;
Feng CHEN
;
Yu-Feng FENG
;
Yong-Ping SONG
;
Ping ZHU
Author Information
1. Henan Tumor Hospital, Henan Institute of Hematology. Zhengzhou 450008, Henan Province, China. zhzhfuyuewen@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Bone Marrow Transplantation;
Complementarity Determining Regions;
genetics;
Graft vs Host Disease;
genetics;
immunology;
Immunoglobulin Variable Region;
genetics;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Molecular Sequence Data;
Receptors, Antigen, T-Cell;
genetics
- From:
Journal of Experimental Hematology
2009;17(4):999-1004
- CountryChina
- Language:Chinese
-
Abstract:
This study was purpused to analyze the characteristics of T cell receptor repertoire in target organs of murine graft-versus-host after haploidentical bone marrow transplantation (hiBMT) and the molecular characteristics of complementarity determining region3 (CDR3) repertoires of monoclonal T cell in liver, skin and ileum in murine after hiBMT. Murine haploidentical BMT model was established, CDR3-size spectratyping was used to study TCRBV repertoires in recipient liver, skin, ileum, spleen and a group of CDR3 molecules was obtained from GVHD-target tissues. The results showed that GVHD occurred as early as days 14 after transplantation and was proven by histology in liver, skin and ileum. A number of new monoclonal and oligoclonal T cells emerged in GVHD-target tissue. 45 CDR3 molecules had six C'-terminal motifs, which obtained from liver, skin, ileum in different times after hiBMT. It is concluded that target organs of murine graft-versus-host disease after hiBMT emerged a number of clonal or oligoclonal T cells, part of this T cell clones commonly uses some conserved CDR3 motifs and may recognize similar antigen.
