Comparison of STR-PCR and FISH value for monitoring chimerism after sex-mismatched allogeneic hematopoietic stem cell transplantation.
- Author:
You-Wen QIN
1
;
Ying JIANG
;
Xiao-Rui WANG
;
Li-Ping WAN
;
Shi-Ke YAN
;
Chun WANG
Author Information
1. Department of Hematology, The First People Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Child;
Female;
Hematopoietic Stem Cell Transplantation;
methods;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Male;
Microsatellite Repeats;
Middle Aged;
Polymerase Chain Reaction;
methods;
Transplantation Chimera;
Transplantation, Homologous;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(4):1016-1020
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to compare the significance of multiplex short tandem repeat polymerase chain reaction (STR-PCR) and fluorescent in situ hybridization (FISH) for monitoring chimerism after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT). The chimerism of bone marrow or peripheral blood cells from 38 patients was analyzed by STR-PCR and FISH on days 14, 28 and at 3 months after allo-HSCT. The results indicated that on day 14, the complete chimerism (CC) was detected in 14 of 30 cases by STR-PCR and in 8 of 30 cases by FISH (p > 0.05). On day 28, the CC was detected in 26 of 31 cases by STR-PCR and in 15 of 31 cases by FISH (p < 0.01). At 3 months, the CC was observed in 22 of 24 cases by STR-PCR and 17 of 24 cases by FISH (p > 0.05). 14 cases were found to have a graft rejection or relapse among 28 cases which were continuously monitored more than 3 months post the transplants. Donor cell decrease in 9 of 14 cases was proved by FISH alone. It is concluded that FISH is more sensitive than STR-PCR in early monitoring chimerism status of post-transplant and in predicting graft rejection or disease relapse.
