Expression of anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in idiopathic thrombocytopenic purpura.
- Author:
Wen-Jie XIA
1
;
Xin YE
;
Yong-Shui FU
;
Xiu-Zhang XU
;
Yang-Kai CHEN
;
Hao-Qiang DING
;
Jing DENG
;
Guang-Ping LUO
;
Ru XU
Author Information
1. Institute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou, 510095, Guangdong Province, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antibodies, Anti-Idiotypic;
blood;
Antigens, Human Platelet;
immunology;
Child;
Child, Preschool;
Female;
HLA Antigens;
immunology;
Humans;
Infant;
Infant, Newborn;
Male;
Middle Aged;
Platelet Glycoprotein GPIIb-IIIa Complex;
immunology;
Platelet Glycoprotein GPIb-IX Complex;
immunology;
Platelet Membrane Glycoproteins;
immunology;
Purpura, Thrombocytopenic, Idiopathic;
blood;
immunology;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(4):1032-1035
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the expression of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in idiopathic thrombocytopenic purpura (ITP), 45 patients with ITP were selected in this study. An easy PCR-SSP assay was used to detect single-nucleotide polymorphisms or deletion in HPA and HLA systems. The anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate were tested with a solid phase ELISA. The results indicated that the anti-platelet glycoprotein specific antibodies were detected in plasma or platelet eluate of 45 patients, among which anti-GPIIb/IIIa/and anti-GpIb/IX were most common. Both the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies were found in plasma of 11 patients. Pedigree investigation in 2 patients (case 37 and case 40) was carried out, the results showed that anti-platelet glycoprotein specific antibodies and anti-HLA antibodies detected in 2 patients closely related to incompatibility with platelet antigens and HLA antigens in parents. In conclusion, the results suggested that detection of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate in combination with investigation of clinical manifestation of patients is important for diagnosis of idiopathic thrombocytopenic purpura.
