The effect of leukocyte depletion by filtration on the quality of apheresis platelets.
- Author:
Yang YU
1
;
Qian FENG
;
Ting ZHANG
;
Chun-Ya MA
;
Xiao-Juan ZHANG
;
Guo-Feng GE
;
Zi-Lin LIN
;
Ji-Chun PAN
;
De-Qing WANG
;
Qun LUO
;
Ya-Ping TIAN
Author Information
1. Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Filtration;
Humans;
Leukapheresis;
instrumentation;
Platelet Count;
Plateletpheresis;
instrumentation;
methods
- From:
Journal of Experimental Hematology
2009;17(4):1067-1070
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of leukocyte depletion by filtration on the quality of apheresis platelets. 20 units of donor apheresis platelets were randomly selected and were preserved with agitation at 20 - 24 degrees C for 24 - 96 hours, then were filtered on polyester flatbed filters. The platelet concentration, mean platelet volume (MPV), volume of apheresis platelets, leukocyte count, pH value, lactate dehydrogenase (LDH) concentration, K(+) concentration and CD62p expression level on surface of platelet membrane, were detected before and after filtration, as well as the rate of leukocyte depletion and platelet loss were calculated. The results showed that the leukocyte count after filtration was remarkably lower than that before filtration (p < 0.001), and the rate of leukocyte depletion was 99.97%. Platelet loss was approximately 8%, and obviously lower than that of the national standard (p < 0.001). MPV, pH value, K(+) and LDH concentration were not significantly different before and after filtration. Compared with platelets before filtration, CD62p expression level after filtration slightly decreased (p > 0.05). CD62p expression on surface of platelet membrane in perfusion fluid obtained from filter plate was obviously higher than that before filtration (p < 0.05). MA of platelet after filtration slightly decreased (p > 0.05). It is concluded that leukocyte and partial activated platelets can be removed efficiently by using polyester flatbed filters, and platelet loss is very low. Filtration does not adversely affect coagulation activity of the platelets in vitro. Apheresis platelets after filtration can fulfil quality requirements to prevent infection of cytomegalovirus and HLA alloimmunization.
