Interaction between mixed-lineage leukemia and Menin proteins.
- Author:
Lin LIU
1
;
Ben-Shang LI
;
Qi-Dong YE
;
Ying-Yi HE
;
Jing-Yan TANG
Author Information
1. Department of Hematology and Oncology, Shanghai Children Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
- Publication Type:Journal Article
- MeSH:
Chromosome Mapping;
DNA-Binding Proteins;
Genetic Vectors;
HEK293 Cells;
Homeodomain Proteins;
Humans;
Leukemia;
genetics;
Myeloid-Lymphoid Leukemia Protein;
genetics;
Protein Interaction Mapping;
Proto-Oncogene Proteins;
genetics;
Transfection
- From:
Journal of Experimental Hematology
2009;17(5):1130-1134
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the subcellular localization of mixed-lineage leukemia (MLL) and Menin proteins, and to explore the interaction between these two proteins. The recombinant eukaryotic cell expression vectors of pcDNA3.1-myc-MLL and pCMV-flag-Menin were constructed respectively, and transfected into the HEK293T cells. Immunofluorescence technique was used to observe the subcellular localization of the two proteins. Co-immunoprecipitation and Western blot methods were applied to evaluate the expression and interaction of the two proteins. The results showed that MLL and Menin proteins could be co-localized in cell nuclei, and the study of binding in vivo revealed that MLL protein could be detected in the immunoprecipitation complex of anti-FLAG, while Menin proteins could also be found in the immunoprecipitation complex of anti-MYC. It is concluded that MLL and Menin proteins co-localized in cell nuclei have same location and the interaction exists between MLL and Menin proteins.
