Expression of genes psma6 and slc25a4 in patients with acute monocytic leukemia.
- Author:
Yin-Xia CHEN
1
;
Wen-Ping WANG
;
Peng-Yu ZHANG
;
Wang-Gang ZHANG
;
Jie LIU
;
Xiao-Rong MA
Author Information
1. Department of Clinical Hematology, The Second Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China. chenyx204@163.com
- Publication Type:Journal Article
- MeSH:
Adenine Nucleotide Translocator 1;
genetics;
metabolism;
Adult;
Bone Marrow;
metabolism;
Female;
Humans;
Leukemia, Monocytic, Acute;
genetics;
metabolism;
Male;
Proteasome Endopeptidase Complex;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2009;17(5):1168-1173
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the expression levels of genes psma6 and slc25a4 in bone marrow of patients with acute monocytic leukemia and their correlation with clinical features and prognosis. The expression levels of genes psma6 and slc25a4 in AML-M5 leukemia cells, normal blood cells and non-leukemia cells were detected by real-time quantitative RT-PCR and compared each other. The expression levels of psma6-encoding protein P27K was assayed by using immunohistochemistry method. The results showed that the expression levels of psma6 mRNA in AML-M5 leukemia cells was lower than that in non AML-M5 leukemia cells, non-leukemia cells and normal blood cells. The results obtained by immunohistochemistry assay were consistent with above-mentioned results. The expression level of psma6 in AML-M5 patients with complete remission was higher than that in AML-M5 patients without remission. The expression level of P27K protein in AML-M5 and AL correlated to leukocyte count in peripheral blood and LDH content. The overexpression of slc25a4 mRNA was found in AML-M5, but there was no significant difference in slc25a4 mRNA expression between the patients with complete remission and those without remission. It is concluded that the expression level of psma6 is probably a new prognostic indicator of acute monocytic leukemia, slc25a4 may be a novel gene of antigen associated with acute monocytic leukemia.
