Fluorescence in situ hybridization on bone marrow smear in the detection of cytogenetic aberrations of multiple myeloma.
- Author:
Xiao-Wei WANG
1
;
Jian-Yong LI
;
Li-Juan CHEN
;
Si-Xuan QIAN
;
Ming HONG
;
Chun QIAO
;
Jian-Fu ZHANG
;
Wei XU
;
Hua LU
;
Jia-Ren XU
Author Information
1. Department of Hematology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Bone Marrow;
pathology;
Chromosome Aberrations;
Chromosomes, Human, Pair 8;
genetics;
Female;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Male;
Middle Aged;
Multiple Myeloma;
genetics;
pathology
- From:
Journal of Experimental Hematology
2009;17(5):1230-1233
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish the technique of interphase fluorescence in situ hybridization (I-FISH) used on smear of bone marrow directly, and to develop a new method for detection of the molecular cytogenetics in multiple myeloma (MM). After a series of treatment, fixation and digestion of the bone marrow smear as the carrier, the chromosome 8 centromere probe were used in I-FISH for molecular cytogenetics detection. At the same time, differences were compared in the results between the new method and the conventional I-FISH. The results showed that there was no statistically significant difference of proportion of various signals in non-hematologic malignancies when detected with the two methods (p>0.05). In bone marrow smear I-FISH, 8 out of 19 cases (42.1%) had abnormality of chromosome 8, including 5 cases with -8 (26.3%) and 3 cases with +8 (15.8%). It is concluded that the I-FISH on smear of bone marrow is characterized by convenience, economy and accuracy. Therefore, it can be used for research of molecular cytogenetics in MM.
