Mechanism of C-reactive protein on proliferation of multiple myeloma U266 cells.
- Author:
Yi-Rong YANG
1
;
Ling-Juan HUANG
;
Yan-Ping MA
;
Yu-Jin LU
;
Lin-Hua YANG
;
Yong-An ZHOU
Author Information
1. Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
C-Reactive Protein;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
HSP90 Heat-Shock Proteins;
metabolism;
Humans;
Inhibitor of Apoptosis Proteins;
metabolism;
Multiple Myeloma;
metabolism;
pathology;
RNA, Messenger;
genetics
- From:
Journal of Experimental Hematology
2009;17(5):1242-1245
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the mechanism of C-reactive protein (CRP) on proliferation of U266 cells. The human multiple myeloma cell line U266 was incubated with human CRP (0, 5, 10, 20 mg/L) for 24 hours, then the proliferation level of U266 cells was detected by using blood analyser. The mRNA expressions of survivin and HSP90alpha were examined by RT-PCR. The results showed that the proliferation ratio was increased, as compared with the control group (p<0.05); furthermore, the mRNA levels of survivin and HSP90alpha were up-regulated in proportion to the increased CRP concentrations. There was significant correlation between expression of survivin and HSP90alpha (r=0.737, p<0.0001) in incubated cells. It is concluded that CRP can stimulate the proliferation of MM cells directly by up-regulating the expression of survivin and HSP90alpha in MM cells. CRP can be regarded as a potential target for MM treatment.
