Modulation of adhesion molecule expression on T cells in bone marrow after in vivo rhG-CSF application.
- Author:
Ming-Rui HUO
1
;
Ying-Jun CHANG
;
Xiang-Yu ZHAO
;
Xiao-Hua LUO
;
Xiao-Jun HUANG
Author Information
1. Institute of Hematology & People Hospital, Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Bone Marrow;
Bone Marrow Cells;
immunology;
metabolism;
Bone Marrow Transplantation;
immunology;
methods;
Cell Adhesion Molecules;
metabolism;
Female;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Humans;
Living Donors;
Male;
Middle Aged;
Recombinant Proteins;
T-Lymphocytes;
immunology;
metabolism;
Young Adult
- From:
Journal of Experimental Hematology
2009;17(5):1316-1320
- CountryChina
- Language:Chinese
-
Abstract:
The aim of study was to investigate the modulation effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on adhesion molecule expression of memory T lymphocyte in the bone marrow grafts. rhG-CSF was administered in 41 donors by subcutaneous injection for 5 consecutive days. Bone marrow grafts were collected on day 4. The percentages of CD4+ and CD8+, and the expressions of CD49d, CD54, CD62L and CD11a on donor T cells of steady state-bone marrow grafts (SS-BM, n=11) and rhG-CSF primed bone marrow (G-BM, n=30) were analyzed by using multi-color flow cytometry. The results indicated that the percentages of CD4+ and CD8+ T cells were significantly lower in G-BM than those in SS-BM (p<0.05). There were no significant differences in the percentages of memory CD4+ and CD8+ T cells between SS-BM and G-BM (p>0.05). The expressions of CD49d on CD4+ and CD8+T cells were significantly lower in G-BM than that in SS-BM (p<0.05). Compared with SS-BM, the expressions of CD54 on CD4+, memory CD4+ T cells and CD8+ T cells were significantly lower in G-BM (p<0.05). The expressions of CD62L on CD4+ and CD8+ T cells and memory T cells were all significantly lower in G-BM (p values were all less than 0.001). The expressions of CD11a on CD4+, memory CD4+ T cells were significantly lower in G-BM than that in SS-BM (p<0.05). There were no significant differences in the expression of CD11a on CD8+, memory CD8+ T cells between SS-BM and G-BM (p>0.05). It is concluded that the expression of cell adhesion molecules on the CD4+ and CD8+ T cells in G-BM is down-regulated after rhG-CSF treatment of healthy donors.
