Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines.

Yu Hua Wong; Wai Yan Tan; Chin Ping Tan; Kamariah Long; Kar Lin Nyam

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Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines.

Author: Yu Hua WONG ¹ ; Wai Yan TAN ¹ ; Chin Ping TAN ² ; Kamariah LONG ³ ; Kar Lin NYAM ¹
Author Information

1. Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia.
2. Department of Food Technology, Faculty of Food Science and Technology,Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
3. Malaysian Agricultural Research & Development Institute (MARDI) PO Box 12301, 50774 Kuala Lumpur, Malaysia.
Publication Type:Journal Article
Keywords: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; HeLa; Human breast cancer; Human cervical cancer; Human colon cancer; Human lung cancer; Sulforhodamine B assay
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(Suppl 1):S510-5
CountryChina
Language:English
Abstract:
OBJECTIVETo examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines.
METHODSThe in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope.
RESULTSThe kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected.
CONCLUSIONSKSE and KSO could be potential sources of natural anti-cancer agents. Further investigations on using kenaf seeds for anti-proliferative properties are warranted.