Genetic diversity and complexity of Plasmodium falciparum infections in Lagos, Nigeria.

Muyiwa K Oyebola; Emmanuel T Idowu; Yetunde A Olukosi; Bamidele A Iwalokun; Chimere O Agomo; Olusola O Ajibaye; Monday Tola; Adetoro O Otubanjo

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Genetic diversity and complexity of Plasmodium falciparum infections in Lagos, Nigeria.

Author: Muyiwa K OYEBOLA ^{1
,

2} ; Emmanuel T IDOWU ³ ; Yetunde A OLUKOSI ⁴ ; Bamidele A IWALOKUN ⁴ ; Chimere O AGOMO ⁴ ; Olusola O AJIBAYE ⁴ ; Monday TOLA ⁴ ; Adetoro O OTUBANJO ³
Author Information

1. Parasitology and Bioinformatics, Faculty of Science University of Lagos, Nigeria
2. Malaria Research Laboratory, Nigeria Institute of Medical Research, Lagos, Nigeria.
3. Parasitology and Bioinformatics, Faculty of Science University of Lagos, Nigeria.
4. Malaria Research Laboratory, Nigeria Institute of Medical Research, Lagos, Nigeria.
Publication Type:Journal Article
Keywords: Antigenic markers; Diversity; Drug trials; Multiplicity of infections; Recrudescence
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(Suppl 1):S87-91
CountryChina
Language:English
Abstract:
OBJECTIVETo analyse the genetic diversity of Plasmodium falciparum (P. falciparum) using msp-1 and msp-2 as antigenic markers.
METHODSParasite DNA was extracted from 100 blood samples collected from P. falciparum-positive patients confirmed by microscopy, and followed by PCR-genotyping targeting the msp-1 (block2) and msp-2 (block 3) allelic families.
RESULTSAll the families of msp-1 (K1, MAD20 and R033) and msp-2 (FC27 and 3D7) locus were observed. Results revealed that K1 (60/100) was the most predominant genotype of msp-1 allelic family followed by the genotypes of MAD20 (50/100) and R033 (45/100). In the msp-2 locus, FC27 genotype (62/100) showed higher frequency than 3D7 genotype (55/100). The allelic families were detected either alone or in combination with other families. However, no R033/MAD20 combination was observed. Multiplicity of infection (MOI) with msp-1 was higher in the locality of Ikorodu (1.50) than in Lekki (1.39). However, MOI with msp-2 was lower in the locality of Ikorodu (1.14) than in Lekki (1.76). There was no significant difference in the mean MOI between the two study areas (P=0.427).
CONCLUSIONSThe observation of limited diversity of malaria parasites may imply that the use of antigenic markers as genotyping tools for distinguishing recrudescence and re-infections with P. falciparum during drug trials is subjective.