Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: an in vitro study.

Author: Hakan SÖYÜT ¹ ; Elif Duygu KAYA ² ; Sükrü BEYDEMIR ³
Author Information

1. Department of Primary Education, Faculty of Education, Bayburt University, 69000 Bayburt, Turkey.
2. Department of Food Engineering, Faculty of Engineering, Iğdır University, 76000 Iğdır, Turkey.
3. Department of Chemistry, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
Publication Type:Journal Article
Keywords: Cefoperazone sodium; Inhibition; Meropenem trihydrate; Paraoxonase; Piperacillin sodium
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(8):603-609
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1).
METHODShPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography.
RESULTSThe three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive.
CONCLUSIONSOur results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.