Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso, Burkina Faso.

Bamba Sanata; Ouédraogo Abdoul Salam; Sangaré Ibrahim; Zida Adama; Cissé Mamoudou; Karou D Simplice; Simpore Jacques; Guiguemdé T Robert; Hennequin Christophe

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Digestive fungal flora in asymptomatic subjects in Bobo-Dioulasso, Burkina Faso.

Author: Bamba SANATA ^{1
,

2} ; Ouédraogo Abdoul SALAM ³ ; Sangaré IBRAHIM ⁴ ; Zida ADAMA ⁵ ; Cissé MAMOUDOU ⁶ ; Karou D SIMPLICE ⁷ ; Simpore JACQUES ⁷ ; Guiguemdé T ROBERT ⁶ ; Hennequin CHRISTOPHE ⁸
Author Information

1. Department of Parasitology-Mycology, University Hospital Sanou Souro, Bobo-Dioulasso, Burkina Faso
2. Department of Parasitology-Mycology, Higher Institute of Health Sciences, Polytechnic University of Bobo-Dioulasso, Burkina Faso.
3. Department of Bacteriology-Virology, Higher Institute of Health Sciences, Polytechnic University of Bobo-Dioulasso, Burkina Faso.
4. Department of Parasitology-Mycology, University Hospital Sanou Souro, Bobo-Dioulasso, Burkina Faso
5. Faculty of Health Sciences, University of Ouagadougou, Burkina Faso.
6. Department of Parasitology-Mycology, Higher Institute of Health Sciences, Polytechnic University of Bobo-Dioulasso, Burkina Faso.
7. Biomolecular Research Center Pietro Annigoni (CERBA/LABIOGENE), Ouagadougou, Burkina Faso.
8. Service of Parasitology-Mycology, Saint Antoine de Paris Hospital, France.
Publication Type:Journal Article
Keywords: Bobo-Dioulasso; Candida; Mass spectrometry; Matrix-assisted laser desorption ionization-time of flight
From:Asian Pacific Journal of Tropical Biomedicine 2014;4(8):659-662
CountryChina
Language:English
Abstract:
OBJECTIVETo identify Candida species in asymptomatic subjects in Bobo-Dioulasso (Burkina Faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry.
METHODSA cross-sectional study was conducted from January to February 2013 in Bobo-Dioulasso to collect fecal and urine specimens from voluntary donors. Fungal strains were isolated on Sabouraud dextrose agar and analyzed using matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry.
RESULTSA total of 135 samples including stools (78.5%, 106/135) and urine (21.5%, 29/135) were analyzed. The results revealed that fecal specimens contained mainly Candida krusei (C. krusei) (42.5%) followed by Candida albicans (29.3%), Candida glabrata (18.0%) and Candida tropicalis (C. tropicalis) (4.7%). C. krusei (34.6%) was also found to be the most frequently identified in urine samples followed by Candida albicans (27.0%), C. tropicalis (15.4%) and Candida parapsilosis. However, uncommon species such as Candida nivariensis, Candida kefyr, Candida norvegensis, Candida parapsilosis, Candida lusitaniae and Candida robusta were also identified from fecal and urines samples.
CONCLUSIONSThis study noted the emergence of species such as C. krusei, Candida glabrata, Candida parapsiolosis, C. tropicalis, Candida nivariensis, Candida norvegensis, and others. It is an imperative to take into account the existence of these species in the therapeutic management of patients in Bobo-Dioulasso.