Over-expression of VEGF and MMP-9 in residual tumor cells of hepatocellular carcinoma after embolization with lipidol.
10.1007/s11596-013-1077-z
- Author:
Yu-long SHI
1
;
Tao XU
;
Le-ping LI
;
Xiao-ping CHEN
Author Information
1. Department of Gastrointestinal Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, China. shiyl9889@126.com
- Publication Type:Journal Article
- MeSH:
Animals;
Carcinoma, Hepatocellular;
metabolism;
therapy;
Cell Line, Tumor;
Embolization, Therapeutic;
methods;
Ethiodized Oil;
therapeutic use;
Hemostatics;
therapeutic use;
Liver Neoplasms;
metabolism;
therapy;
Matrix Metalloproteinase 9;
metabolism;
Neoplasm, Residual;
metabolism;
Rabbits;
Treatment Outcome;
Up-Regulation;
drug effects;
Vascular Endothelial Growth Factor A;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(1):90-95
- CountryChina
- Language:English
-
Abstract:
The expression and implication of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in residual hepatic tumor cells after lipiodol embolization were investigated. Two weeks after transplantation of VX2 tumor cells into the livers of rabbits, a xenograft model of the human hepatic neoplasm was successfully established. Forty rabbits were randomly divided into control group (n=20) and lipiodol group (n=20). For the control group, 1 mL normal saline was injected through the gastroduodenal artery, whereas 0.3 mL/kg lipiodol was applied for the lipiodol group. One week after embolization, the expression level of VEGF in the plasma was measured by using enzyme-linked immunosorbent assay (ELISA). A three-step immunohistochemical technique (ABC) was employed to detect the protein levels of VEGF and MMP-9 and the quantitative PCR for their mRNA levels was performed in the residual tumor cells. The VEGF in the plasma was significantly higher in the lipiodol group (1.42±0.29 ng/mL) than in the control group (1.12±0.21 ng/mL) (P<0.01). Moreover, the positive rate of VEGF protein in the residual tumor cells was significantly higher in the lipiodol group (62.13%±7.69%) than in the control group (53.16%±9.17%) (P<0.05). Similarly, the MMP-9 expression in the residual tumor cells was higher in the lipiodol group. The mRNA levels of VEGF (2.9313±2.4231) and MMP-9 (3.5721±1.6107) in the lipiodol group were significantly higher than those in the control group (1.5728±0.9453 and 1.7573±1.0641, respectively, P<0.05). Therefore, it was reasonable to speculate that the increased expression of VEGF and MMP-9 in residual hepatic tumor cells and tumor angiogenesis post-embolization would be responsible for the increased metastatic potentiality and invasiveness of these cells.
