Over-expression of LRIG3 suppresses growth and invasion of bladder cancer cells.
10.1007/s11596-013-1081-3
- Author:
Yong QI
1
;
Lei CHANG
;
Heng LI
;
Gan YU
;
Wei XIAO
;
Ding XIA
;
Wei GUAN
;
Yang YANG
;
Bin LANG
;
Kang-li DENG
;
Wei-min YAO
;
Zhang-qun YE
;
Qian-yuan ZHUANG
Author Information
1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Qiy9527@163.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Humans;
Membrane Proteins;
genetics;
metabolism;
Neoplasm Invasiveness;
Up-Regulation;
Urinary Bladder Neoplasms;
metabolism;
pathology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(1):111-116
- CountryChina
- Language:English
-
Abstract:
The purpose of this study was to investigate the impact of leucine-rich repeats and immunoglobulin-like domains 3 (LRIG3) on the biological features of bladder cancer cell lines. The plasmids of over-expressed LRIG3 and the blank plasmid serving as control were transfected into the bladder cancer cell lines, T24, EJ and BIU-87, and the expression levels of LRIG3 mRNA and protein were detected by using real-time PCR and Western blotting. The changes in the cell cycle and apoptosis were examined by using flow cytometry. The invasive ability was measured by Transwell assay, and CCK-8 assays were used to measure the proliferation of cells. As compared with the control group, the LRIG3 mRNA and protein expression levels in LRIG3 cDNA-transfected group were raised significantly (P<0.05). The average number of cells with up-regulated LRIG3 passing through the inserted filter was decreased significantly as compared with the control group (P<0.05). Up-regulation of LRIG3 also could inhibit proliferation and induce apoptosis of T24, EJ and BIU-87 cells. Except BIU-87, the T24 and EJ cells transfected with LIRG3 cDNA were arrested in G(0)/G(1) phase compared to the control group (P<0.05). In conclusion, the over-expression of LRIG3 could influence the cell cycle and invasion, inhibit proliferation and induce apoptosis in the three bladder cancer cell lines.
