Expression of cytokines in mouse hepatitis B virus X gene-transfected model.
10.1007/s11596-013-1092-0
- Author:
Li-fang SUN
1
;
Chuan SHI
;
Lu YUAN
;
Yun SUN
;
Xin-xin YAO
;
Jing-wei MA
;
Chun-mei HUANG
;
Hui-fen ZHU
;
Ping LEI
;
Guan-xin SHEN
Author Information
1. Department of Immunology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. sunnyfang1985@yahoo.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Chemokine CXCL10;
immunology;
Chemokine CXCL9;
immunology;
Cytokines;
immunology;
DNA, Viral;
genetics;
Hepatitis B;
genetics;
immunology;
virology;
Hepatitis B virus;
genetics;
immunology;
Male;
Mice;
Mice, Inbred C57BL;
Mice, Transgenic;
Trans-Activators;
genetics;
Transfection;
methods
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(2):172-177
- CountryChina
- Language:English
-
Abstract:
The expression profile in the mouse hepatitis B virus X (HBx)-transfected model was investigated in order to lay a foundation for further study on the implication of cytokines expression in hepatitis B virus (HBV) infection. Hydrodynamic injection method via the tail vein was used to establish the animal HBx-transfected model. By using microassay, the differential expression of gene in each group was analyzed, which was further confirmed by using real-time PCR and semi-quantitative PCR. Most of chemokine genes such as Ccl2, Ccl5, Ccl9, MIG and IP-10 were up-regulated in the HBx-transfected mouse model versus the control mice, which was coincided with the microarray results. Western blotting and immunohistochemistry were applied to detect the expression of MIG and IP-10 in the liver tissues. Simultaneously, ELISA was adopted to measure the content of IFN-γ in the liver tissues. DNA microassay revealed that the expression of 611 genes changed in HBx-transfected mice as compared with that in pCMV-tag2B-transfected mice, and most of the screened chemokines were up-regulated (including MIG and IP-10). Additionally, IFN-γ protein levels were increased by 20.7% (P<0.05) in pCMV-tag2B-HBx-transfected mice as compared with the untreated mice. IFN-γ protein levels were reduced by 53.9% (P<0.05) in pCMV-tag2B-transfected mice as compared with the untreated mice, which was consistent with the up-regulation of MIG and IP-10. It was suggested HBx transfection could induce the expression of MIG and IP-10 in the liver tissues, which might play the roles in HBV-related liver immunity and cytokines-mediated antiviral effect.
