TGF-β1-induced synthesis of collagen fibers in skeletal muscle-derived stem cells.
10.1007/s11596-013-1104-0
- Author:
Yan-hua CHEN
1
;
Yun-long PENG
;
Yang WANG
;
Yuxiong WENG
;
Tao LI
;
Yan ZHANG
;
Zhen-bing CHEN
Author Information
1. Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. chenyanhua1977@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Differentiation;
drug effects;
physiology;
Cells, Cultured;
Fibrillar Collagens;
biosynthesis;
Male;
Myoblasts, Skeletal;
cytology;
drug effects;
metabolism;
Rats;
Rats, Sprague-Dawley;
Stem Cells;
cytology;
drug effects;
metabolism;
Transforming Growth Factor beta1;
pharmacology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(2):238-243
- CountryChina
- Language:English
-
Abstract:
The aim of this study was to investigate the mechanism of deposition of extracellular matrix induced by TGF-β1 in skeletal muscle-derived stem cells (MDSCs). Rat skeletal MDSCs were obtained by using preplate technique, and divided into four groups: group A (control group), group B (treated with TGF-β1, 10 ng/mL), group C (treated with TGF-β1 and anti-connective tissue growth factor (CTGF), both in 10 ng/mL), and group D (treated with anti-CTGF, 10 ng/mL). The expression of CTGF, collagen type-I (COL-I) and collagen type-III (COL-III) in MDSCs was examined by using RT-PCR, Western blot and immunofluorescent stain. It was found that one day after TGF-β1 treatment, the expression of CTGF, COL-I and COL-III was increased dramatically. CTGF expression reached the peak on the day 2, and then decreased rapidly to a level of control group on the day 5. COL-I and COL-III mRNA levels were overexpresed on the day 2 and 3 respectively, while their protein expression levels were up-regulated on the day 2 and reached the peak on the day 7. In group C, anti-CTGF could partly suppress the overexpression of COL-I and COL-II induced by TGF-β1 one day after adding CTGF antibody. It was concluded that TGF-β1 could induce MDSCs to express CTGF, and promote the production of COL-I and COL-III. In contrast, CTGF antibody could partially inhibit the effect of TGF-β1 on the MDSCs by reducing the expression of COL-I and COL-III. Taken together, we demonstrated that TGF-β1-CTGF signaling played a crucial role in MDSCs synthesizing collagen proteins in vitro, which provided theoretical basis for exploring the methods postponing skeletal muscle fibrosis after nerve injury.
