Protective effect of mouse 2.5s nerve growth factor on PC12 cells from injury induced by 2, 5-hexanedione.
- Author:
Ling-cong SUN
1
;
La-ju XIA
;
Xiang-ping MENG
;
Li LIU
;
Xing-hua GAO
;
Guo-cheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; DNA Fragmentation; drug effects; Dose-Response Relationship, Drug; Electrophoresis, Agar Gel; Flow Cytometry; Hexanones; toxicity; Mice; Nerve Growth Factors; pharmacology; PC12 Cells; Rats; Tumor Suppressor Protein p53; biosynthesis
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(3):154-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether the nerve growth factor has protective effects on PC12 cells from injury induced by 2, 5-hexanedione.
METHODSWith PC12 cells as the model of neurons, different concentrations of NGF were added into the culture of PC12 cells. Then cell viability was tested with MTT. The DNA fragment was observed with agarose gel electrophoresis. The apoptosis ratio was tested with flow cytometry (FACS). The p53 protein was detected with western blot. The differences among the groups were compared.
RESULTSCell viabilities were increased with the increase of the concentrations of NGF (P < 0.05). The DNA fragment, the apoptosis ratio and the expression of p53 were all decreased with the increase of the concentrations of NGF (P < 0.05).
CONCLUSIONThe NGF might have direct nutritional effects on PC12 cells, and protect them from injury induced by 2, 5 HD. Moreover, it might also have anti-apoptosis effect to some extent.
