The role of JWA in N-methyl-N'-nitro-N-nitrosoguanidine induced human bronchial epithelial cell apoptosis.
- Author:
Yan-qiong XU
1
;
Ai-ping LI
;
Rui CHEN
;
Jian-wei ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Bronchi; cytology; drug effects; metabolism; Cells, Cultured; Dose-Response Relationship, Drug; Epithelial Cells; drug effects; metabolism; Heat-Shock Proteins; biosynthesis; physiology; Humans; Intracellular Signaling Peptides and Proteins; physiology; Methylnitronitrosoguanidine; toxicity; Signal Transduction; Transcription Factors; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(4):205-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role and possible mechanism of JWA in N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced human bronchial epithelial (HBE) cell apoptosis.
METHODSThe cell growth inhibition rate was detected by MTT, the cell apoptosis was measured by Hoechst staining, the expression of JWA protein was detected by Western blot, and the potential binding protein of JWA proximal promoter was detected by Southwestern assay.
RESULTSMNNG treatment of HBE cells for 24 hours induced apoptosis with significant dose-effect relationship and in this course the expression of JWA protein was elevated. The 2.0 microg/ml MNNG treated cells for 24 hours activated nuclear transcription factor expression that specifically bound to JWA proximal promoter.
CONCLUSIONThat MNNG treatment activates nuclear transcription factor binding to JWA proximal promoter may be involved in intracellular apoptosis associated signal pathway.
