Clinical significance of peripheral blood circular DNA level measurements in patients with acute myocardial infarction.
- Author:
Yunqiang XU
1
;
Bei LIU
;
Zhaoxia ZHAO
;
Guihua WANG
;
Xianghui ZHU
;
Shiyang PAN
;
Dan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Case-Control Studies; DNA, Circular; blood; Female; Humans; Male; Middle Aged; Myocardial Infarction; blood; diagnosis
- From: Chinese Journal of Medical Instrumentation 2012;36(6):456-458
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore clinical value of circular DNA in acute myocardial infarction.
METHODSVenous blood (2 ml/head) of 40 healthy control and 40 patients with acute myocardial infarction within 48h of onset of illness and convalescent period was collected. The level of plasma circular DNA was detected by duplex real-time polymerase chain reaction assay. The levels of myocardial enzyme spectrum and cardiac troponin T (cTnT) were detected by biochemistry method.
RESULTSThe level of circular DNA in control group and group of acute myocardial infarction before treatment was (21.5 +/- 10.7) ng/ml and (253.6 +/- 45.7) ng/ml, respectively (P = 0.000). The levels of serum myocardial enzyme spectrum and cTnT before treatment in patients with acute myocardial infarction were significantly higher than those of control group (P < 0.05). The level of circular DNA after treatment in patients with acute myocardial infarction was significantly decreased compared with that before treatment (P = 0.000), the levels of myocardial enzyme spectrum and cTnT were also significantly reduced (P < 0.05). There was significant correlation between the level of circular DNA and those of CK-MB and cTnT, r = 0.613, 0.654, P = 0.032, 0.021.
CONCLUSIONCircular DNA can be used as a marker of sensitively reflecting myocardial cell injury.
