Secretory expression and biological function of IGFBP-3 in eukaryotic cells.
- Author:
Chen WU
1
;
Yaojun SHAN
;
Minji ZOU
;
Jiaxi WANG
;
Donggang XU
Author Information
1. College of Life Sciences, Hebei University, Baoding 071002, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Base Sequence;
Carcinoma, Renal Cell;
metabolism;
pathology;
Humans;
Insulin-Like Growth Factor Binding Protein 3;
genetics;
secretion;
Kidney Neoplasms;
metabolism;
pathology;
Molecular Sequence Data;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
physiology;
Transfection;
Tumor Cells, Cultured
- From:
Chinese Journal of Biotechnology
2008;24(4):558-562
- CountryChina
- Language:Chinese
-
Abstract:
The cDNA of Insulin-like growth factor binding protein 3 was subcloned into a eukaryotic secretory expression vector pSectagA to construct pSectag-IGFBP3. Human renal cell carcinoma (RCC) 786-0 cells were transfected with pSectag-IGFBP3 using lipofectamine 2000. After 48 h, the secretory IGFBP-3 was tested and identified by western blotting. Meanwhile, Annexin V-EGFP stain was used to analyze the apoptosis of 786-0 cells induced by IGFBP-3. Secretory IGFBP-3 protein could express successfully in the 786-0 cells and the expressed IGFBP-3 directly displayed an apoptotic effect on the host cells. This work provides a basis for further study on the apoptosis-inducing mechanism of IGFBP-3 and the development of a new anti-tumor drug.
