Phage resistance of Corynebacterium crenatum conferred by the restriction and modification system cglI.
- Author:
Yongfei HU
1
;
Tiemin LI
;
Zhiyong YANG
;
Bo ZHANG
;
Yu LI
Author Information
1. College of Life Science, Liaoning University, Shenyang 110036, China.
- Publication Type:Journal Article
- MeSH:
Amino Acids;
biosynthesis;
Bacterial Proteins;
genetics;
Bacteriophages;
growth & development;
Corynebacterium;
genetics;
virology;
Corynebacterium glutamicum;
genetics;
metabolism;
DNA Restriction-Modification Enzymes;
genetics;
Fermentation;
Galectins;
genetics;
Recombination, Genetic;
Transformation, Bacterial
- From:
Chinese Journal of Biotechnology
2008;24(5):760-765
- CountryChina
- Language:Chinese
-
Abstract:
In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PCR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cglI into C. crenatum. Results showed that the recombinant strains possessed strong phage-resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contamination in amino acid fermentation industry.
