Global expression profiling of Saccharomyces cerevisiae: metabolic remodeling in post-log phase.
- Author:
Yanrui YE
1
;
Yuqian TANG
;
Hongyun CHEN
;
Suiping ZHENG
;
Li PAN
;
Ying LIN
Author Information
1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
- Publication Type:Journal Article
- MeSH:
Amino Acids;
biosynthesis;
Ethanol;
metabolism;
Gene Expression Profiling;
Gene Expression Regulation, Fungal;
Ketoglutaric Acids;
metabolism;
Oligonucleotide Array Sequence Analysis;
Saccharomyces cerevisiae;
genetics;
growth & development;
metabolism;
Succinic Acid;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(6):962-967
- CountryChina
- Language:Chinese
-
Abstract:
For the purpose of revealing the mechanism of the reduction of yeasts ethanol production rate after entrance of post-log phase, we used microarray to study expression profiles of the yeast Saccharomyces cerevisiae during the transition from mid-log growth phase to post-log growth. The results demonstrate that the global pattern of gene expression is very stable during the mid-log phase. However, a dramatic metabolic remodeling was found when the yeast entries post-log phase, during which many of amino acid synthesis and metabolism related genes are up-regulated, moreover, ion transport, energy generation and storage related genes are also up regulated during this phase, while a large number of genes involved in transposition and DNA recombination are repressed. Central metabolic pathways also engage in metabolic remodeling, within which the genes involved in succinate and a-ketoglutarate synthesis pathways are up regulated, accordance with those of amino acid synthesis and metabolism. These results demonstrate that the increasing demand for amino acids in post-log phase lead to a metabolic transition into TCA cycle and glyoxylate cycle, which subsequently reduce the ethanol production rate. This suggests a global insight into the process of yeast ethanol fermentation.
