Cloning and characterization of the chloramphenicol resistant gene of Serratia marcescens strain KMR-3.
- Author:
Xiuling JI
1
;
Lianbing LIN
;
Shenrong JING
;
Yunlin WEI
Author Information
1. Biotechnology Research Center of Kunming University of Science and Technology, Kunming 650224, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Chloramphenicol Resistance;
genetics;
Cloning, Molecular;
Molecular Sequence Data;
Serratia marcescens;
classification;
genetics
- From:
Chinese Journal of Biotechnology
2008;24(6):1016-1021
- CountryChina
- Language:Chinese
-
Abstract:
Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug resistance transporter, Bcr/CflA subfamily of Serratia proteamaculans 568. Regulation elements including promoter, terminator, Shine-Dalgarno (SD) sequence and transcription start site also were identified.
