Optimization of bacterial cellulose fermentation medium and observation of bacterial cellulose ultra-micro-structure.
- Author:
Ruiqin WU
1
;
Shuangkui DU
;
Zhixi LI
;
Xiaohui XING
;
Dongyan SHAO
;
Yanli FAN
;
Bo LI
;
Xueliang ZHANG
;
Lijun BU
Author Information
1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
Cellulose;
biosynthesis;
ultrastructure;
Culture Media;
chemistry;
Fermentation;
Gluconobacter;
cytology;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(6):1068-1074
- CountryChina
- Language:Chinese
-
Abstract:
In order to improve the yield of bacterial cellulose (BC), the fermentation medium of BC-producing strain J2 (Gluconobacter) was optimized, and BC ultra-micro-structure was observed. Initially, Plackett-Burman design was employed to evaluate eight variables which were relevant to BC production. Three statistically significant parameters including yeast extract, ZnSO4, ethanol were selected and other 5 variables were not significant (P > 0.05). The optimized levels of three variables were defined by Box-Behnken design and response surface methodology (RSM). BC ultra-micro-structure was observed by scanning electron microscope (SEM) with cotton cellulose as comparison. The results indicated that the BC yield under the optimum fermentation medium was 11.52 g/100 mL, which was as 1.35 times as that under the original fermentation medium. The SEM photos manifested that bacterial cellulose ribbon, with a diameter less than 0.1 microm, was less than cotton cellulose ribbon. The bacteria inside the cellulose net were eliminated after the NaOH treatment.
