Propionic acid fermentation by Propionibacterium freudenreichii CCTCC M207015 with a fibrous-bed bioreactor.
- Author:
Xiaohai FENG
1
;
Bo WU
;
Xiaobo SHEN
;
Hong XU
Author Information
1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China.
- Publication Type:Journal Article
- MeSH:
Bioreactors;
microbiology;
Fermentation;
Glucose;
metabolism;
Propionates;
metabolism;
Propionibacterium;
classification;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(6):1075-1079
- CountryChina
- Language:Chinese
-
Abstract:
The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a Fibrous-bed bioreactor (FBB). The FBB was constructed by packing spiral cotton fibrous and immobilized into a bioreactor. By applying this bioreactor to propionic acid fermentation, the propionic acid yield had a significant improvement and reached 20.41 g/L, compared with the cell-free culture of 14.58 g/L (40 g/L of glucose). At the same time, the glucose exhausting time decreased from 120 h to 60 h. Batch fermentations at various glucose concentrations were carried out with FBB. Based on the analysis of the time course of production, fed-batch fermentation was also applied to produce propionic acid with FBB, the maximal propionic acid yield reached 45.91 g/L, and the proportion of propionic acid to total acids was about 72.31%.
