Changes of the metabolism of anti-oxidation competence of endothelial cells induced from mesenchymal stem cells after fluid shear stress loading.
- Author:
Siyuan YANG
1
;
Yingkang SHI
;
Changxue WU
;
Ke DIAN
;
Jiang WU
;
Wentong MENG
;
Yuchun WANG
Author Information
1. Department of Cardio-thoracic Surgery, West China Hospital of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antioxidants;
metabolism;
Bone Marrow Cells;
cytology;
physiology;
Cell Differentiation;
physiology;
Cells, Cultured;
Endothelial Cells;
cytology;
metabolism;
Female;
Male;
Mesenchymal Stromal Cells;
cytology;
physiology;
Oxidation-Reduction;
Rats;
Rats, Sprague-Dawley;
Stress, Mechanical
- From:
Journal of Biomedical Engineering
2008;25(3):616-620
- CountryChina
- Language:Chinese
-
Abstract:
This study examined the effects of flow shear stress on the bio-capacity of the endothelial cells' induced from mesenchymal stem cells (MSCs). After cultivating the SD rat mesenchymal stem cells in vitro, we exposed them under different intensity of flow shear stress and induced these cells to endothelial cells. The variations of total anti-oxidation competence (T-AOC) and quantity of nitrogen monoxide (NO) were tested. The results showed that shear stress has an enhanced effect on the T-AOC and NO of endothelial cells induced from MSCs in an intensity-dependent manner. Flow shear stress could provide a protective action on the in vitro induction of endothelial cells, thus formulating a theoretical foundation for the therapeutics of ischemic heart diseases and vascular tissue engineering.
