The study of apoptosis and mechanism of cells exposed to steep pulse.
- Author:
Huan LIU
1
;
Liling TANG
;
Caixin SUN
;
Yan MI
;
Chenguo YAO
;
Chengxiang LI
;
Yuanliang WANG
Author Information
1. Bioengineering College of Chongqing University, Chongqing 400044, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
radiation effects;
Calcium;
metabolism;
Electricity;
Electromagnetic Fields;
Hepatocytes;
cytology;
pathology;
Humans;
Liver Neoplasms;
metabolism;
pathology;
Microscopy, Confocal;
Tumor Cells, Cultured
- From:
Journal of Biomedical Engineering
2008;25(3):637-641
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was designed to study the apoptosis and related mechanism of adherent liver tumor cells (SMMC-7721) and adherent normal liver cells (HL-7702) when they were exposed to the steep pulse generated by the steep pulse apparatus for tumor treatment. The results showed that the steep pulse of 200 V could induce tumor cells apoptosis. The tumor cells presented with their apoptosis when they were exposed to the steep pulse from 200 V to 250 V. Laser scanning confocal microscopy was used to make a real time study of calcium burst when the adherent tumor cells were exposed to the steep pulse. The results showed:On the condition of no extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V decreased more evidently. On the condition of existing extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased little; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V reduced little, too. Maybe the change of calcium burst in the tumor cells is the mechanism of apoptosis when cells are exposed to the steep pulse.
