Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC.
- Author:
Saiyu CHENG
1
;
Huaizhen RUAN
;
Zhong YANG
;
Xigui WU
Author Information
1. Department of Neurobiology, The Third Military Medical University, Chongqing Institute of Neuroscience, Chongqing 400038, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
Animals;
Cloning, Molecular;
DNA, Complementary;
genetics;
Gene Transfer Techniques;
Genetic Therapy;
Genetic Vectors;
genetics;
Glial Cell Line-Derived Neurotrophic Factor;
genetics;
Neurons;
cytology;
Rats;
Recombinant Proteins;
genetics;
Stem Cells;
cytology
- From:
Journal of Biomedical Engineering
2008;25(3):642-646
- CountryChina
- Language:Chinese
-
Abstract:
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
